产品说明
一般描述
HMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.
免疫原
Recombinant protein corresponding to mouse HMGB1.
应用
Research Sub Category
Chromatin Biology
Cell Cycle, DNA Replication & Repair
Use Anti-HMGB1 Antibody, clone HMG1-5H6 (Hamster Monoclonal Antibody) validated in WB, IF, FC to detect HMGB1 also known as High-mobility group box 1.
Immunofluorescence Analysis: 10 µg/mL from a representative lot detected HMGB1 in NIH/3T3 cells. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)
Flow Cytometry Analysis: A representative lot detected HMGB1 in RAW264.7 cells treated with Cytofix/Cytoperm. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
质量
Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected HMGB1 on 10 µg of NIH/3T3 cell lysate.
目标描述
~ 30 kDa observed
外形
Purified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
NIH/3T3 cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 300 100 |
生物来源 | hamster |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | HMG1-5H6, monoclonal |
species reactivity | rat, human, mouse |
technique(s) | flow cytometry: suitable immunofluorescence: suitable western blot: suitable |
NCBI登记号 | NP_034569 |
UniProt登记号 | P09429 |
运输 | wet ice |
Gene Information | human ... HMGB1(3146) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich