Anti-HPK1 Antibody

产品说明

一般描述

Hematopoietic progenitor kinase 1 (HPK1), otherwise known as MEKKK 1 and MAP4K1, is a serine/threonine kinase belonging to the Ste20 (Sterile-20) family. It is predominantly expressed in hematopoietic cells and is involved in sigaling pathways requiring MAP kinase activation; HPK1 directly upstream of MEKK1. HPK1 forms signaling complexes with a number of adaptor proteins including Nck, SLP-76, and Grb2 family of proteins; these proteins may facilitate the tethering of HPK1 to membrane receptors such as the epidermal growth factor receptor. However, HPK1 may also interact with adaptor proteins to induce transcription. For example, interaction with CRKL, and Crk adaptor proteins activates the c-Jun N-terminal kinase (JNK) pathway leading to gene expression. HPK1 may mediate cell growth, cytokine signaling, and cell death.

特异性

Other homologies: Mouse and Rat (86% sequence homoloogy).
This antibody recognizes HPK1 at the N-terminus.

免疫原

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human HPK1.

应用

Anti-HPK1 Antibody detects level of HPK1 & has been published & validated for use in Western Blotting, IHC(P).
Research Category
Signaling
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected HPK1 in human tonsil tissue.
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected HPK1 on 10 µg of Raji cell lysate.

目标描述

~97kDa observed. The calculated molecular weight is 91 kDa, however HPK1 has been shown as a ~97 kDa band in western blots. (Hu, M., et al. (1996). Genes Dev. 10:2251-2264.)

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Raji cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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