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Anti-Lin-28B, clone 15G3 Antibody

品牌
Sigma-Aldrich
货号
MABE885
规格纯度
clone 15G3, from mouse
参考价格
5352.52 *本价格含增值税费
促销
服务
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数量
-+
产品名称:
Anti-Lin-28B, clone 15G3 Antibody
Protein lin-28 homolog B, Lin-28B
产品介绍:

产品说明

一般描述

Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

特异性

This antibody does not cross react with Lin-28A.

免疫原

Recombinant protein corresponding to human Lin28B.

应用

Anti-Lin-28B, clone 15G3 | MABE885 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Research Sub Category
Nuclear Receptors
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Lin28B in 10 µg of NTera2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate.

目标描述

~30 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~20 kDa

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆15G3, monoclonal
species reactivityhuman
technique(s)immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001004317
UniProt登记号Q6ZN17
运输wet ice
Gene Informationhuman ... LIN28B(389421)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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