您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Anti-NMDAR1 Antibody, clone 54.1

Anti-NMDAR1 Antibody, clone 54.1

品牌
Sigma-Aldrich
货号
MAB363
规格纯度
clone 54.1, Chemicon®, from mouse
参考价格
6526.19 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-NMDAR1 Antibody, clone 54.1
N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1
产品介绍:

产品说明

一般描述

Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.

特异性

Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.

免疫原

Epitope: Extracellular loop between transmembrane regions III & IV.
Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.

应用

Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
Research Category
Neuroscience
Immunocytochemistry:
A previous lot of this antibody was used in IC.

RIA:
A previous lot of this antibody was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA.

Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

Optimal working dilutions must be determined by end user.
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by immunohistochemistry on rat brain and spinal cord tissue.

Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord

目标描述

103-116 kDa

外形

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.

分析说明

Control
Rat brain and spinal cord tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆54.1, monoclonal
species reactivityXenopus, rat, human, monkey
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
同位素/亚型IgG2a
NCBI登记号NM_000832.5
UniProt登记号Q05586
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品