Anti-phospho Beclin Antibody (Thr119)

产品说明

一般描述

The Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson′s and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.

特异性

This antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.

免疫原

KLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.
Epitope: BH3 domain

应用

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Research Sub Category
Apoptosis - Additional
Western Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.

目标描述

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

外形

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Human prostate tumor tissue lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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