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位置:首页 > 品牌 > Sigma-Aldrich > Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5

Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5

品牌
Sigma-Aldrich
货号
MABE940
规格纯度
clone 6G12C5, 1 mg/mL, from rat
参考价格
6694.05 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5
Histone H3.3
产品介绍:

产品说明

一般描述

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

免疫原

KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr11.

应用

This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11).
Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa cell lysate.

Dot Blot (Specificity) Analysis: 0.2 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells undergoing interphase and metaphase (Prof. Taro Tachibana, Cell Engineering Corporation.).

ELISA Analysis: A representative lot detected phospho Histone H3 (Thr11) in a panel of Histone H3 peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).

Western Blotting Analysis: A representative lot detected phospho Histone H3 (Thr11) in HCT116 cell lysates, which were incubated for a period of time with either nocodazole or UV. The same lot detected phospho Histone H3 (Thr11) in HeLa cell lysates, which were incubated for a period of time with nocodazole. Additionally, the same lot detected phospho Histone H3 (Thr11) in tsFT210 whole cell extracts after released G2/M syncronization (Prof. Taro Tachibana, Cell Engineering Corporation.).

Western Blotting Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in nocodazole treated HeLa extract (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.)

ELISA Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in a panel of various phosphorylated H3 peptides (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in mitotic DM4 cells (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).

质量

Evaluated by Western Blotting in nocodazole treated and untreated HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa acid extract.

目标描述

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

联系

Replaces: MABE468

外形

Protein G Purified
Format: Purified

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆6G12C5, monoclonal
species reactivityhuman
浓度1 mg/mL
technique(s)ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_002098
UniProt登记号P84243
运输wet ice
Gene Informationhuman ... H3F3B(3021)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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