Anti-Potassium Channel Kv1.1 Antibody, clone K36/15

产品说明

一般描述

Potassium voltage-gated channel subfamily A member 1, better known as Potassium Channel Kv1.1, or Kcna1 belongs to the Shaker potassium channel group responsible for mediating ion permeability of excitable membranes. Conformational changes as a result of voltage changes across the cell membrane varies the permeability of this multi-pass transmembrane channel to potassium. Mutations of the Shaker potassium channel gene in Drosophila and the KCNA1 gene for its mammalian orthologue, Kv1.1, reduce the probability of action potential failure thereby causing hyperexcitability near axon branch points and nerve terminals, thereby leading to uncontrolled movements and recapitulating the episodic ataxia-1 (EA1) symptoms in human patients. Further, the axonal Kv1 channel activity may regulate synaptic transmission by modulating the action potential waveform, duration, firing pattern, and spread of depolarization into axonal branches.

特异性

Other homologies: Human (94% sequence homology).

免疫原

Linear peptide corresponding to 18 amino acids from the extracellular domain of rat Potassium Channel Kv1.1.

应用

Western Blotting Analysis: 4 µg/mL from a representative lot detected Potassium Channel Kv1.1 in 10 µg of rat brain membrane tissue lysate.

Immunofluoresense Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Tiffany, A. M., et al. (2000). J Cell Biol. 148(1):147-158.).

Immunhistochemistry Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Wenzel. H. J., et al (2007). Epilepsia. 48(11):2023-2046.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detect Potassium Channel Kv1.1 in wild type mouse hippocampus tissue, and demonstrated a loss of signal in Potassium Channel Kv1.2 knock out mouse hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).
Anti-Potassium Channel Kv1.1 Antibody, clone K36/15 is a highly specific mouse monoclonal antibody, that targets Kv1.1 & has been tested in Western blotting, Immunofluorescence & IHC.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Potassium Channel Kv1.1 in 10 µg of mouse brain tissue lysate.

目标描述

~65 kDa observed. The calculated molecular weight of this protein is 56 kDa However, it can run higher due to glycosylation (Deal, K. K., et al. (1994). J Neurosci. 14(3 Pt 2):1666-1676.).

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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