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Anti-Ribeye Antibody, clone 3D12.1

品牌
Sigma-Aldrich
货号
MABN804
规格纯度
clone 3D12.1, from mouse
参考价格
5352.52 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Ribeye Antibody, clone 3D12.1
C-terminal-binding protein 2, CtBP2, Ribeye
产品介绍:

产品说明

一般描述

Human C-terminal-binding protein 2 (CtBP2) and Ribeye (UniProt P56545-1 and P56545-2, respectively) are two proteins encoded by the CTBP2 gene (Gene ID 1488) in human, they are produced as a result of alternative splicing. The C-terminal 425-amino acid sequence of Ribeye (aa 561-985) is identical to that of CtBP2 (aa 21-445), however the first 20 amino acids at the CtBP2 N-terminal end is replaced with a 560-amino acid sequence in the case of Ribeye. Ribeye is a protein specific to synaptic ribbons. Ribbon synapses are high-performance synapses built by certain nonspiking sensory neurons, such as photoreceptors, retinal bipolar cells, and inner ear hair cells. The main site of exocytosis of these neurons is characterized by unique presynaptic structures called the synaptic ribbons that associate with large numbers of synaptic vesicles primed for exocytosis and has a direct impact on synaptic signaling. Ribeye is composed of an N-terminal A domain and a C-terminal B domain that is largely identical to CtBP2. The A domain unique to Ribeye has a structural role at the synaptic ribbon, while the B domain of Ribeye possesses lysophosphatidic acid (LPA) acyltransferase (LPAAT) activity that catalyzes the generation of the highly active signaling phospholipid phosphatidic acid (PA) from LPA. LPAAT-deficient Ribeye mutant does not recruit PA-binding proteins to synaptic ribbons, whereas wild-type RIBEYE supports PA-dependent binding, The B domain also contains an NADH-binding subdomain (NBD), which is reported to compete against Arf1 for Arf-GTPase activating protein-3 (ArfGAP3) binding. Ribeye therefore functions as an Arf1 positive regulator at the synaptic ribbon by preventing the inactivation of Arf1 by ArfGAP3.

特异性

Specific for CTBP2 spliced variant Ribeye. Does not react with the canonical CtBP2 isoform.

免疫原

GST-tagged recombinant protein corresponding to the N-terminal region of human Ribeye.
Epitope: N-terminal region

应用

Anti-Ribeye Antibody, clone 3D12.1, Cat. No. MABN804, is a highly specific mouse monoclonal antibody, that targets Ribeye and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ribeye in 10 µg of SH-SY5Y cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Ribeye in human cardiac myocytes and human cerebellum tissues.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ribeye in 10 µg of HeLa cell lysate.

目标描述

~106 kDa observed (109.2 kDa calculated).

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆3D12.1, monoclonal
species reactivityhuman
technique(s)immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_073713
UniProt登记号P56545-2
运输dry ice
Gene Informationhuman ... CTBP2(1488)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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