产品说明
一般描述
TS (EC:2.1.1.45), a cytosolic enzyme, is a dimmer of two identical monomers of about 36kDa. The enzyme provides the sole intracellular de novo source of thymidylate and plays a crucial role in DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dump) and its conversion to deoxythymidine monophosphate (dTMP). Therefore, TS is primarily active in proliferating and metabolic active cells. TS is a central target of the widely used antineoplastic agent 5-Fluorouracil (5-FU) and thus also of the Xeloda, which is enzymatically activated to 5-FU. TS is inactivated by a covalent complex formation with 5-FdUMP and methylenetetrahydrofolate. Literature indicates that expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas with low TS expression predicting better response to 5-FU and survival.
特异性
Antibody MAB4130 recognizes a 36 kDa protein identified as Thymidylate Synthase [TS], which is a target for the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. Expression of TS is associated with response to 5-fluorouracil (5-FU) in human breast, colorectal, gastric, head, and neck carcinomas.
免疫原
Recombinant Thymidylate Synthase.
应用
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Thymidylate Synthase Antibody, clone TS106 (Mouse Monoclonal Antibody) validated in ELISA, FC, IF, IHC, IHC(P), IP, WB to detect Thymidylate Synthase also known as Thymidylate synthase.
Research Category
Epigenetics & Nuclear Function
ELISA:
A previous lot of this antibody was used in ELISA.
Flow Cytometry:
A previous lot of this antibody was used in FC.
Immunofluorescence:
A previous lot of this antibody was used in IF.
Immunoprecipitation:
A 10 μL/mg concentration of a previous lot was used on protein lysate.
Immunohistochemistry (Frozen and Paraffin sections):
1:50 - 1:100 (with incubation for 30-60 minutes at room temperature). TS106 can benefit from antigen retrieval with either EDTA pH 8.0 or 10mM citrate buffer pH 6.0 .
Western Blotting:
1:200 - 1:400. Recognizes a 36KDa band.
Optimal working dilutions must be determined by end user.
质量
Evaluated by Western Blot on MCF7 lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected THYMIDYLATE SYNTHASE on 10 μg of MCF7 lysates.
目标描述
36 kDa
外形
Format: Purified
Purified mouse monoclonal IgG1 in 0.02M PBS with 0.25M NaCl, pH=7.6, 0.1% sodium azide.
Protein A purified
储存及稳定性
Stable for 1 year at 2-8ºC from date of receipt.
分析说明
Control
Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
克隆 | TS106, monoclonal |
species reactivity | human, mouse |
species reactivity (predicted by homology) | rat |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable flow cytometry: suitable immunofluorescence: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_001071.1 |
UniProt登记号 | P04818 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich