Anti-WHSC1/NSD2 Antibody, clone 29D1

产品说明

一般描述

Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1), Nuclear SET domain containing protein 2 (NSD2) is a histone lysine methyltransferase that is deregulated in a subgroup of multiple myelomas. NSD proteins have been described to serve as oncogenes; however, little is known about their targets in transcriptional repression or activation. NSD2 is overexpressed in multiple myelomas via translocation between the NSD2 locus on chromosome 4 and the immunoglobulin locus on chromosome 14. NSD2 is under the regulatory control of this immunoglobulin locus. Further research is required to elucidate its roles and mechanisms in cancer.

免疫原

GST-tagged recombinant protein corresponding to human WHSC1/NSD2.

应用

Detect WHSC1/NSD2 using this Anti-WHSC1/NSD2 Antibody, clone 29D1 validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation.
Western Blot Analysis: 0.5-1 µg/mL from a representative lot detected WHSC1/NSD2 in 10 µg of HeLa nuclear extract and human placenta tissue lysate.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in transfected Gal4-reporter/293T cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in KMS11 cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

质量

Evaluated by Western Blot in HCT116 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected WHSC1/NSD2 in 10 µg of HCT116 cell lysate.

目标描述

~152 kDa observed. There are seven isoforms ranging between ~30 and ~152 kDa Different lysates may indicate different isoforms in Western Blots.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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