产品说明
一般描述
AURA/AURKA (aurora kinase A) is mapped to human chromosome 20q13.2. It is also termed as STK15 (serine/threonine kinase 15). This gene codes for a protein of 403 amino acids with regulatory and catalytic domains. AURA is overexpressed in several malignancies like invasive carcinomas of ovary, head, neck and breast.
Human Aurora kinase A (GenBank Accession No. NM_003600), amino acids 2- 403 with N-terminal HIS6-tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
AURA/AURKA (aurora kinase A) modulates cell cycle checkpoints and plays important roles in centrosome division, cytoskeleton and cytokinesis. It sustains the integrity of chromosomes after DNA damage. The catalytic domain of AURKA possesses a serine/threonine kinase activity.
单位定义
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 50 kDa |
| 包装 | pkg of 10 μg |
| technique(s) | activity assay: suitable inhibition assay: suitable |
| NCBI登记号 | NM_003600 |
| UniProt登记号 | O14965 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... AURKA(6790) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
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