产品说明
一般描述
Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.
应用
Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used in a study to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases. Active human cathepsin L has also been used in a study to investigate the role of cathepsin B and L activity in the serum during the human aging process.
单位定义
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37℃
外形
Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
法律信息
FreeStyle is a trademark of Invitrogen Corp.
基本信息
| NACRES | NA.54 |
产品性质
| 重组 | expressed in FreeStyle™ 293-F cells |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 36 kDa |
| NCBI登记号 | NM_001912 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CTSL(1514) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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