产品说明
一般描述
Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.
应用
Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.
单位定义
One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37℃
外形
Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
法律信息
FreeStyle is a trademark of Invitrogen Corp.
基本信息
| NACRES | NA.54 |
产品性质
| 重组 | expressed in FreeStyle™ 293-F cells |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| specific activity | ≥436 pmol/min-μg |
| 分子量 | 37 kDa |
| NCBI登记号 | NM_001333.2 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CTSL2(1515) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H360D |
| 预防措施声明 | P201 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
| 储存分类代码 | 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
