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Glucokinase from human pancreas

品牌
Sigma-Aldrich
CAS
9001-51-8
货号
SRP6046
规格纯度
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
参考价格
3049.16 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
9001-51-8
Glucokinase from human pancreas
Hexokinase, pancreatic
Pancreatic glucokinase
Hexokinase
产品介绍:

产品说明

一般描述

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

单位定义

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30℃. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30℃ for 30 min.

外形

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

基本信息

NACRESNA.32

产品性质

生物来源human pancreas
重组expressed in E. coli
测定≥80% (SDS-PAGE)
形式liquid
分子量 53.1 kDa
包装pkg of 10 μg
浓度1 mg/mL
杂质 Endotoxin, tested
NCBI登记号P35557
UniProt登记号P35557
运输dry ice
储存温度−70℃
Gene Informationhuman ... GCK(2645)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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