Human CRISPR Brunello Knockout Library

产品说明

一般描述

The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD). The library is designed to be compact and efficient to maximize screening efficiency and performance.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

应用

• Functional Genomics/Target Validation
• Unbiased wholed genome forward genetic screening
• Validated positive and negative controls
• Set up and optimization of screen assay

特点和优势

• Focus on your research, and we will generate your lentivirus screening library
• Use CRISPR nucleases to knockout protein-coding genes to assess their function.
• Human Genes targeted 19,114
• Compact library of ~ four gRNAs per gene (76,441 total)
• Total Controls 1000(pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO for sources of Cas9

原理

In a CRISPR KO screen, Cas9 introduces double-strand breaks at locations specified by a gRNA. When the endogenous non-homologous end-joining (NHEJ) DNA repair system corrects these breaks, this often leads to the introduction of frame-shift mutations that effectively knock out the gene. Thus, the power of CRISPR for genome engineering, coupled with the ability to perform large-scale, whole-genome loss-of-function (LOF) screening, has allowed breakthroughs in identifying gene pathways in drug resistance and disease.

法律信息

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

产品性质

安全信息