产品说明
应用
Useful for enzyme assay and Western blot.
生化/生理作用
Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer.
重悬
Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in E. coli |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 2.0-3.5 nmol/min·mg (GTPase-Glo) |
| 分子量 | ~23 kDa |
| 浓度 | 0.1 μg/μL |
| 登记号 | NM_033360 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... KRAS(3845) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H360D |
| 预防措施声明 | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
