产品说明
一般描述
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
About the Cleavage Tag:This plasmid does not contain a protease cleavage site.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
序列
To view sequence information for this product, please visit the product page
分析说明
To view the Certificate of Analysis for this product, please visit www.oxgene.com
法律信息
FLAG is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.85 |
产品性质
| 标签 | FLAG® tagged |
| 形式 | buffered aqueous solution |
| 分子量 | size 6174 bp |
| 复制起点 | pUC (500 copies) |
| 肽切割 | no cleavage |
| 肽标签位置 | C-terminal |
| 启动子 | Promoter name: CMV Promoter activity: constitutive Promoter type: mammalian |
| 菌种筛选 | kanamycin |
| 哺乳动物细胞筛选 | puromycin |
| 报告基因 | none |
| 运输 | ambient |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
