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位置:首页 > 品牌 > Sigma-Aldrich > PSF-CMV-PURO-COOH-FLAG®-C-TERMINAL FLAG® TAG MAMMALIAN PLASMID

PSF-CMV-PURO-COOH-FLAG®-C-TERMINAL FLAG® TAG MAMMALIAN PLASMID

品牌
Sigma-Aldrich
货号
OGS3423
规格纯度
plasmid vector for molecular cloning
参考价格
5636.88 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
PSF-CMV-PURO-COOH-FLAG®-C-TERMINAL FLAG® TAG MAMMALIAN PLASMID
expression vector
molecular cloning vector
vector
snapfast vector
plasmid vector
cloning vector
plasmid
产品介绍:

产品说明

一般描述

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.

About the Cleavage Tag:This plasmid does not contain a protease cleavage site.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.85

产品性质

标签FLAG® tagged
形式buffered aqueous solution
分子量size 6174 bp
复制起点pUC (500 copies)
肽切割no cleavage
肽标签位置C-terminal
启动子Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
菌种筛选kanamycin
哺乳动物细胞筛选puromycin
报告基因none
运输ambient
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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