PTP1B (1-321)/PTPN1 Active from rat

产品说明

一般描述

Protein tyrosine phosphatase, catalytic domain (amino acids. 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

应用

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

单位定义

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.

外形

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

产品性质