产品说明
一般描述
Human SMURF 1 (GenBank Accession No. NM_020429), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 70 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected insect cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| specific activity | ≥0.0055 pmol/min-μg |
| 分子量 | 70 kDa |
| 包装 | pkg of 20 μg |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_020429 |
| UniProt登记号 | Q9HCE7 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... SMURF1(57154) |
Sigma-Aldrich
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