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SMURF2 Active human

品牌
Sigma-Aldrich
货号
SRP0228
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
参考价格
7548.98 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
SMURF2 Active human
SMAD specific E3 ubiquitin protein ligase 2
MGC138150
E3 ubiquitin ligase SMURF2
产品介绍:

产品说明

一般描述

Human SMURF 2 (GenBank Accession No. NM_022739), amino acids 130-end, with N-terminal DDDDK tag (FLAG), MW = 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥80% (SDS-PAGE)
形式aqueous solution
specific activity≥0.00049 pmol/min-μg
分子量 72 kDa
包装pkg of 20 μg
储存条件avoid repeated freeze/thaw cycles
浓度>0.02 mg/mL
NCBI登记号NM_022739
UniProt登记号Q9HAU4
运输dry ice
储存温度−70℃
Gene Informationhuman ... SMURF2(64750)

Sigma-Aldrich

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