Anti-AFP Mouse mAb (1G7)

产品说明

一般描述

Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.

免疫原

A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein

包装

100 μg in Glass bottle

应用

ELISA (see coments)

Immunoblotting (1-5 µg/ml)

Immunocytochemistry (10 µg/ml)

警告

Toxicity: Regulatory Review (Z)

外形

In PBS, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
HepG2 cells
Negative Control
293T cells

其他说明

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息