Anti-FANCD2 Antibody, clone 10F8.1

产品说明

一般描述

Fanconi anemia group D2 protein (UniProt: Q9BXW9; also known as Protein FACD2) is encoded by the FANCD2 (also known as FACD) gene (gene ID: 2177) in human. FACD2 is a nuclear protein that is required for maintenance of chromosomal stability. It is highly expressed in fetal oocytes, and in hematopoietic cells of the fetal liver and bone marrow. High expression is also observed in germinal center cells of the spleen, tonsil, and reactive lymph nodes and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, larynx and cervix. FACD2 promotes accurate and efficient pairing of homologs during meiosis. It is also involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing and may participate in S phase and G2 phase checkpoint activation upon DNA damage. It is concentrated in nuclear foci during S phase and upon genotoxic stress. FACD2 plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. It is phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, it is phosphorylated on serine 222 and serine 1404 by ATM. Phosphorylation on serine 222 is shown to be essential for S-phase checkpoint activation. Mutations in FANCD2 gene are known to cause Fanconi anemia complementation group D2, which results in anemia, leukopenia, and thrombopenia and may cause cardiac, renal, and limb malformations and increase predisposition to malignancies. (Ref.: Shen, Y., et al. (2015). Cancer Cell Microenviron. 2(4): e986).

特异性

Clone 10F8.1 specifically detects human Fanconi anemia group D2 protein. It targets an epitope with in the first 150 amino acids from the N-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to first 150 amino acids of human Fanconi anemia group D2 protein.

应用

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected FANCD2 in human tonsil and human testis tissue sections.

Research Category
Signaling
Anti-FANCD2, clone 10F8.1, Cat. No. MABS1889, is a mouse monoclonal antibody that detects Fanconi anemia group D2 protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected FANCD2 in Jurkat cell lysate.

目标描述

~166 kDa observed; 164.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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