产品说明
一般描述
Recognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
Purified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
This Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) is validated for use in ELISA, Immunoblotting, Frozen Sections, IP, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.
免疫原
bovine GFAP
Bovine Brain
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (0.1-0.5 µg/ml)
Immunoblotting (0.1-0.5 µg/ml)
Frozen Sections (10-50 µg/ml)
Immunoprecipitation (2-5 µg/ml)
Paraffin Sections (10-50 µg/ml, see comments)
警告
Toxicity: Standard Handling (A)
外形
In PBS.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Human or rat brain tissues
其他说明
Tohyama, T., et al. 1993. Am. J. Pathol.142, 871.
Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
Product is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin′s-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | rat |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 2.2B10, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | mouse, bovine, human, rat |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG2a |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich