产品说明
一般描述
Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.
Recognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
免疫原
A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
HL-60 cells, HeLa cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | 1G7, monoclonal |
形式 | liquid |
不包含 | preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich