Calcineurin Autoinhibitory Peptide-Calbiochem

产品说明

一般描述

Corresponds to a C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin. Specific calcineurin inhibitor. Does not inhibit CaM kinase II, protein phosphatase I or 2A. Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated activity.
Specific calcineurin inhibitor. Corresponds to the C-terminal domain (residues 457-482) of the calmodulin-binding domain of calcineurin (PP2B). Inhibits Mn²⁺-stimulated calcineurin activity (IC₅₀ = 10 µM using ³²P-myosin light chain as a substrate) but has no effect on Ni²⁺-stimulated enzyme activity. Does not inhibit CaM kinase II, protein phosphatase 1 or 2A.

包装

250 μg in Plastic ampoule
Yes

生化/生理作用

Product does not compete with ATP.
Primary Target
Mn²⁺-stimulated calcineurin activity
Reversible: no
Cell permeable: no
Target IC₅₀: 10 µM using 32P-myosin light chain as a substrate

警告

Toxicity: Standard Handling (A)

序列

H-Ile-Thr-Ser-Phe-Glu-Glu-Ala-Lys-Gly-Leu-Asp-Arg-Ile-Asn-Glu-Arg-Met-Pro-Pro-Arg-Arg-Asp-Ala-Met-Pro-OH

外形

Supplied as a trifluoroacetate salt.

重悬

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 2 months at -20°C.

其他说明

Perrino, B.A., et al. 1995. J. Biol. Chem. 270, 340.
Yokoyama, Y., and Wang, J.H. 1994. FEBS Lett.337, 128.
Yokoyama, N., et al. 1993. Arch. Biochem. Biophys.300, 615.
Matsui, H., et al. 1991. Biochem. Int.24, 1119.
Hashimoto, Y., et al. 1990. J. Biol. Chem.265, 1924.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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