Histone Deacetylase 8, His·Tag^®, Human Recombinant, S. frugiperda

产品说明

一般描述

Full length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.

外形

In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN^®-20 detergent, pH 8.0.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Waltregny, D., et al. 2005. FASEB J.19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

产品性质

安全信息