产品说明
一般描述
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
组分
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
法律信息
Imprint is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
组份列表
组份可单独销售
| 货号 | 组份 |
| I8896 | IGEPAL® CA-630, for molecular biology |
| S5150 | Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture |
| W4502 | Water, Nuclease-Free Water, for Molecular Biology |
| A8456 | 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC) |
| P8340 | Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution |
| R4642 | Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) |
安全信息
| 象形图 |      |
| 警示用语： | Danger |
| 危险声明 | H226 - H271 - H290 - H302 - H314 - H336 - H410 |
| 预防措施声明 | P210 - P273 - P280 - P301 + P312 + P330 - P303 + P361 + P353 - P305 + P351 + P338 + P310 |
| 危险分类 | Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Skin Corr. 1B - STOT SE 3 |
| 靶器官 | Central nervous system |
| 储存分类代码 | 5.1A - Strongly oxidizing hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | 100.4 °F |
| 闪点(C) | 38 ℃ |
Sigma-Aldrich
m.cnreagent.com
