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位置:首页 > 品牌 > Sigma-Aldrich > Anti-BimEL (pS69) human / (pS65) rat Antibody

Anti-BimEL (pS69) human / (pS65) rat Antibody

品牌
Sigma-Aldrich
货号
AB3579
规格纯度
Chemicon®, from rabbit
参考价格
7701.24 *本价格含增值税费
促销
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数量
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产品名称:
Anti-BimEL (pS69) human / (pS65) rat Antibody
产品介绍:

产品说明

一般描述

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

特异性

Rat BimEL when phosphorylated at serine 65. Human BimEL when phosphorylated at serine 69. Mouse BimEL (100% conserved) has not been tested, but is expected to react.

免疫原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimEL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.

应用

Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.

质量

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a BimEL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

外形

Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

储存及稳定性

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

分析说明

Control
Hek293T co-expressing rat BimEL and active MEKK1 (MEKK1δ).

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, rat
species reactivity (predicted by homology)mouse (100% homologous)
manufacturer/tradenameChemicon®
technique(s)western blot: suitable
NCBI登记号NM_006538.3
NM_138621.3
NM_138622.2
NM_138623.2
NM_138624.2
NM_138626.2
NM_138627.2
NM_207002.2
NM_207003.1
UniProt登记号O43521
运输dry ice
Gene Informationhuman ... BCL2L11(10018)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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