Anti-G_sα-Subunit, C-Terminal (385-394) Rabbit pAb

产品说明

一般描述

Recognizes both large and small forms of G_sα-subunit. Does not cross-react with G_iα-1-, G_iα-2-, G_iα-3-, or G_oα-subunits.
Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa G_sα subunit protein.
Anti-G_sα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of G_sα-subunit. It is validated for use in Western blotting.

免疫原

a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian G_sα subunit, conjugated to KLH

包装

50 μL in Glass bottle

应用

Immunoblotting (1:1000)

警告

Toxicity: Standard Handling (A)

外形

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-70°C).

分析说明

Positive Control
G_sα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or G_sα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)

其他说明

Does not cross-react with G_iα-1, G_iα-2, G_iα-3, or G_oα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_sα, G_iα-1, G_Iα-2, G_iα-3, and G_oα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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