产品说明
特异性
Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.
免疫原
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}
应用
Research Category
Cell Structure
Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.
Research Sub Category
Integrins
Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)
ELISA/RIA: 1:1000
Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit
Immunoprecipitation: 5 μL precipitates alpha2/beta1 from 5 x 106 cells.
Immunohistochemistry: 1:500
Optimal working dilutions must be determined by end user.
目标描述
160kDa
外形
Neat rabbit antiSerum. Liquid with 0.05% sodium azide
Unpurified
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Skin (basement membrane), High grade ovarian tumors
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | mouse, chicken, hamster, rat, human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable |
NCBI登记号 | NM_002203.3 |
UniProt登记号 | P17301 |
运输 | wet ice |
Gene Information | human ... ITGA2(3673) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich