Anti-Growth Associated Protein 43 Antibody, phosphoSer 41

产品说明

一般描述

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

特异性

GAP-43 phosphoSer41. The labeling of the GAP-43 protein (~43kDa) in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. In some tissues the antibody may recognize a lower molecular weight band. This band is also detected by a pan GAP-43 antibody.

免疫原

Epitope: phosphoSer 41
Synthetic peptide corresponding to amino acids surrounding the phosphoSer41 of rat GAP-43. This is the site phosphorylated by protein kinase C.

应用

This Anti-Growth Associated Protein 43 Antibody, phosphoSer 41 is validated for use in WB for the detection of Growth Associated Protein 43.
Western blot: 1:1,000 (43KDa band).

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience

外形

Affinity purified immunoglobulin. Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl with 100 mg/mL BSA and 50% glycerol.

储存及稳定性

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

分析说明

Control
Synthetic peptide corresponding to amino acids surrounding the phosphoSer41 of rat GAP-43.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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