产品说明
一般描述
GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
特异性
GAP-43 phosphoSer41. The labeling of the GAP-43 protein (~43kDa) in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. In some tissues the antibody may recognize a lower molecular weight band. This band is also detected by a pan GAP-43 antibody.
免疫原
Epitope: phosphoSer 41
Synthetic peptide corresponding to amino acids surrounding the phosphoSer41 of rat GAP-43. This is the site phosphorylated by protein kinase C.
应用
Research Category
Neuroscience
Research Sub Category
Neuroregenerative Medicine
Signaling Neuroscience
Anti-Growth Associated Protein 43 Antibody, phosphoSer 41 is an antibody against Growth Associated Protein 43 for use in WB.
Western blot: 1:1,000 (43kDa band).
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin in 10 mM HEPES (pH 7.5), 150 mM NaCl with 100 μg/mL BSA and 50% glycerol.
储存及稳定性
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | western blot: suitable |
NCBI登记号 | NM_002045.2 |
UniProt登记号 | P17677 |
运输 | dry ice |
Gene Information | human ... GAP43(2596) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich