Anti-HCN4 Antibody, clone PG2-1A4

产品说明

一般描述

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (UniProt: O70507; also known as Brain cyclic nucleotide-gated channel 3, BCNG-3, HCN4) is encoded by the Hcn4 (also known as Bcng3) gene in murine species. HCN4 is a homo- or hetero-tetrameric multi-pass membrane protein that is detected in a subset of elongated cells in taste buds. It acts as a hyperpolarization-activated ion channel with very slow activation and inactivation. It exhibits weak selectivity for potassium over sodium ions. HCN4 is reported to contribute to the native pacemaker currents in heart that regulates the rhythm of heart beat. It may also contribute to the native pacemaker currents in neurons. HCN4 is activated by cAMP and cAMP binding causes a conformation change that leads to the assembly of an active tetramer and induces channel opening. This channel may mediate responses to sour stimuli.

特异性

Clone PG2-1A4 detects murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4). It targets an epitope within 70 amino acids from the C-terminal region.

免疫原

GST-tagged recombinant fragment corresponding to 70 amino acids from the C-terminal region of murine Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4).

应用

Immunofluorescence Analysis: A representative lot detected HCN4 in Immunofluorescence applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39).

Immunohistochemistry Analysis: A 1:100-500 dilution from a representative lot detected HCN4 in Mouse (C57BL/6) retina tissues (Courtesy of Prof. Dr. Frank Müller, Institute of Complex Systems, Cellular Biophysics (ICS-4), Forschungszentrum Jülich, Germany).

Electron Microscopy Analysis: A representative lot detected HCN4 in Electron Microscopy applications (Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

Immunohistochemistry Analysis: A representative lot detected HCN4 in Immunohistochemistry applications (Cichy, A., et. al. (2015). J Neurosci. 35(9):4025-39; Mataruga, A., et. al. (2007). J Comp Neurol. 502(6):1123-37).

Western Blotting Analysis: A representative lot detected HCN4 in Western Blotting applications (Muller, F., et. al. (2003). Eur J Neruosci. 17(10):2084-96).

Anti-HCN4, clone PG2-1A4 Antibody, Cat. No. MABN1871, is a highly specific rat monoclonal antibody that targets murine HCN4 and has been tested for use in immunofluorescence, Immunohistochemistry, Electron Microscopy, and Western Blotting.

质量

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.

目标描述

127.41 kDa calculated.

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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