产品说明
特异性
The antibody recognizes native and recombinant proteins.
免疫原
Catalytic domain of MT1-MMP
应用
Research Category
Cell Structure
Immunocytochemistry: 5-10 μg / ml
Western blot: 5 μg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 μg / mg total protein in 500 μl reaction volume.
FACS Analysis: 5 μg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Research Sub Category
MMPs & TIMPs
外形
Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.
储存及稳定性
Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.
分析说明
Control
Immunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable flow cytometry: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NM_004995.2 |
UniProt登记号 | P50281 |
运输 | dry ice |
Gene Information | human ... MMP14(4323) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich