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位置:首页 > 品牌 > Sigma-Aldrich > Anti-Opioid Receptor μ Antibody

Anti-Opioid Receptor μ Antibody

品牌
Sigma-Aldrich
货号
AB5509
规格纯度
Chemicon®, from guinea pig
参考价格
6694.05 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-Opioid Receptor μ Antibody
MOR-1
产品介绍:

产品说明

特异性

Mu Opioid receptor.

免疫原

Synthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor.

应用

Research Sub Category
Neuroinflammation & Pain
Research Category
Neuroscience
Anti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH.
Immunohistochemistry: 1:50-500 See Protocol.

Immunocytochemistry: 1:50-500 See Protocol.

Optimal working dilutions must be determined by the end user.

IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above.

联系

Replaces: AB1774

外形

PBS containing 50% glycerol; Concentration 1.0 mg/ml

储存及稳定性

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源guinea pig
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityrat, mouse, primate
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
NCBI登记号NM_000914.2
NM_001008503.1
NM_001008504.1
NM_001008505.1
UniProt登记号P35372
运输dry ice
Gene Informationhuman ... OPRM1(4988)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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