Anti-Orexin-B Antibody

产品说明

一般描述

Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al., 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al., 1998; DeLecca et al., 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

特异性

Mouse/Rat Orexin-B. Reacts with full length Orexin-B. The AB3100 epitope is not found in Orexin-A. Since Orexins-A and Orexin-B are derived from an unprocessed pre-pro orexins (130 aa), the anti-Orexin-B antibodies may also react with pre-pro orexins.

免疫原

A 13 amino acid peptide mapping near the amino terminus of mouse Orexin-B. The immunogen peptide has 92% similarity with human Orexin-B.

应用

Anti-Orexin-B Antibody detects level of Orexin-B & has been published & validated for use in ELISA & WB.
Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

外形

Affinity purified immunoglobulin. Liquid in PBS, with 0.1% BSA .

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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