Anti-Potassium Channel Kv1.3 Antibody

产品说明

一般描述

Anti-Potassium Channel Kv1.3 Antibody detects level of Potassium Channel Kv1.3 & has been published & validated for use in IH & WB.

特异性

Recognizes a full length Kv1.3 protein. Possibly cross reacts with Kv1.1.

免疫原

GST fusion protein and a C-terminal portion of human Kv1.3 protein (amino acids 471-523) (Accession P22001).

应用

Detect Potassium Channel Kv1.3 using this Anti-Potassium Channel Kv1.3 Antibody validated for use in IH & WB.
Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

联系

Replaces: AB9784

外形

Format: Purified
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 mL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

储存及稳定性

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

分析说明

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For positive control, in Western blot using 20 ng of protein per lane (minigel). For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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