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Anti-TRRAP Antibody, clone 2TRR-1B3

品牌
Sigma-Aldrich
货号
MABE1008
规格纯度
ascites fluid, clone 2TRR-1B3, from mouse
参考价格
5426.21 *本价格含增值税费
促销
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数量
-+
产品名称:
Anti-TRRAP Antibody, clone 2TRR-1B3
Transformation/transcription domain-associated protein, 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog
产品介绍:

产品说明

一般描述

Transformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation.

特异性

The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).

免疫原

Epitope: N-terminal to nuclear localization signal in the TP53-interacting region.
Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.

应用

Research Category
Epigenetics & Nuclear Function
Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412).
Research Sub Category
Histone Modifying Proteins

质量

Evalulated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 µg of HeLa nuclear extract.

目标描述

~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Unpurified
Mouse monoclonal IgG2aκ ascites without preservatives.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆2TRR-1B3, monoclonal
species reactivityhuman
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_001231509
UniProt登记号Q9Y4A5
运输dry ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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