您好,欢迎来到试剂仪器网! [登录] [免费注册]
试剂仪器网
位置:首页 > 品牌 > Sigma-Aldrich > Osteopontin, His·Tag®, Mouse, Recombinant, Mouse

Osteopontin, His·Tag®, Mouse, Recombinant, Mouse

品牌
Sigma-Aldrich
货号
499260
包装型号
规格纯度
参考价格
7379.67 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Osteopontin, His·Tag®, Mouse, Recombinant, Mouse
rmOPN
产品介绍:

产品说明

一般描述

Recombinant, mouse osteopontin fused at the C-terminus to a HisTag sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca2+-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
Recombinant, mouse osteopontin fused at the C-terminus to a HisTag sequence and expressed in NSO-derived Leu17-Asn294 (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
M.W. 65,000 and 30,000 under reducing conditions.

包装

50 μg in Plastic ampoule

生化/生理作用

~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.

重悬

Reconstitute to ≥100 µg/ml with sterile PBS.
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.

其他说明

Xie, Y., et al. 2001. Kidney Int.60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

测定≥95% (SDS-PAGE)
质量水平100
形式lyophilized
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
杂质≤100 pg/μg Endotoxins (pg/μg rmOPN)
运输ambient
储存温度−70℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品