Sulfo-EGS (Ethylene glycol bis(sulfosuccinimidyl succinate))

产品名称：

产品介绍：

产品说明

一般描述

EGS is a water-insoluble, homobifunctional N-hydroxysuccinimide ester (NHS ester) and Sulfo-EGS is its water-soluble analog. The spacer arm contains two cleavable ester sites that may be broken with hydroxylamine, which yields two fragments with terminal amide bonds and the release of ethylene glycol. These reagents are often used for conjugating radiolabeled ligands to cell surface receptors. The water insoluble reagent EGS does not possess a charged group, is lipophilic and, therefore, membrane-permeable and useful for intracellular and intramembrane protein conjugation. EGS is first dissolved in DMSO or DMF and added to the aqueous reaction mixture at a final solvent concentration of 10-20%, to minimize detrimental affects to the protein.

特点和优势

Reactive groups: sulfo-NHS ester (both ends)
Reactive towards: amino groups (primary amines)
Water-soluble; compare to EGS
Membrane-impermeable, allowing for cell surface labeling
Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37℃
Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

注意

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

基本信息

经验(实验)分子式	C₁₈H₁₈O₁₈N₂Na₂S₂
分子量	660.45
MDL编号	MFCD00054988
PubChem化学物质编号	329768789
NACRES	NA.22

产品性质

形式	powder
质量水平	100
分子量	660.45
reaction suitability	reagent type: cross-linking reagent
储存条件	desiccated
溶解性	water: soluble
运输	ambient
储存温度	−20℃
InChI	1S/C18H20N2O18S2.2Na/c21-11-7-9(39(29,30)31)17(27)19(11)37-15(25)3-1-13(23)35-5-6-36-14(24)2-4-16(26)38-20-12(22)8-10(18(20)28)40(32,33)34;;/h9-10H,1-8H2,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2
InChI key	IYBKWXQWKPSYDT-UHFFFAOYSA-L

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H319 - H335
预防措施声明	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable