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位置:首页 > 品牌 > Sigma-Aldrich > ToyoScreen® AF-rProtein A HC-650F Process Development Column

ToyoScreen® AF-rProtein A HC-650F Process Development Column

货号
823432
规格纯度
phase rProtein A, pkg of 5 × 5 mL, 45 μm particle size
参考价格
38975.9 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
ToyoScreen® AF-rProtein A HC-650F Process Development Column
产品介绍:

产品说明

一般描述

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

产品规格

Clean in place with 0.1 M NaOH.

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法律信息

ToyoScreen is a registered trademark of Tosoh Corporation
Toyopearl is a registered trademark of Tosoh Corporation

产品性质

物料aluminum seal
polypropylene and polyethylene frit
产品线ToyoScreen®
specific analyte(s)IgG
包装pkg of 5 × 5 mL
manufacturer/tradenameTosoh 23432
参数1-5 mL/min flow rate
20 mL/min max. flow rate
3 bar max. pressure
technique(s)HPLC: suitable
长度 × 内径3 cm × 14.6 mm
基质 hydroxylated methacrylic polymer
基质活性基团rProtein A phase
粒径45 μm
操作pH2 - 13
容量 >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
>80 g/L adsorption capacity (IgG)(static)
分离技术affinity

Sigma-Aldrich

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