产品说明
一般描述
Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).
包装
1 ml in Plastic ampoule
Packaged under inert gas
生化/生理作用
Product does not compete with ATP.
Reversible: no
Cell permeable: no
警告
Toxicity: Irritant (B)
外形
A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.
重悬
Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.
其他说明
Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C44H47N3O24 |
| 分子量 | 1001.85 |
| MDL编号 | MFCD00036976 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | liquid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze protect from light |
| 荧光 | λex 335 nm (high) λex 362 nm (low) λem 505 nm (high) λem 512 nm (low) |
| 运输 | ambient |
| 储存温度 | −20℃ |
| InChI | 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3 |
| InChI key | UTBYGIDPBQOHNT-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | 188.6 °F - (Dimethylsulfoxide) |
| 闪点(C) | 87 ℃ - (Dimethylsulfoxide) |
Sigma-Aldrich
m.cnreagent.com
