Chaperonin 60 from Escherichia coli

产品说明

应用

Chaperonin 60 from Escherichia coli has been used:

• in mass spectroscopy
• in cryo-electron microscopy imaging as control for testing particle distribution
• as standard in infrared spectrum measurements

包装

1 mg in serum bottle
Package size based on protein content.

生化/生理作用

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles. The chaperonins assist the folding of nascent, organelle-imported or stress-destabilized polypeptides. In vitro, purified GroEL together with purified GroES in the presence of Mg-ATP facilitate refolding and reactivation of denatured proteins, e.g., the photosynthetic enzyme rubisco and the mitochondrial enzyme rhodanese.
The folding activity of a 1:1 molar mixture of GroEL and GroES was tested using urea-denatured rhodanese. At least 2-fold reactivation of rhodanese over the spontaneous reactivation was obtained.

外形

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

基本信息

产品性质

安全信息