产品说明
生化/生理作用
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
组分
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
注意
This product should be stored at -20℃. After preparation, the product can be stored at 2-8℃ for one month, or frozen in aliquots at -20℃ or -70℃ for more extended storage.
外形
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
制备说明
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
分析说明
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
基本信息
| MDL编号 | MFCD00282118 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 效能 | 10-100 ng/mL ED50/EC50 |
| 分子量 | ~11 kDa |
| 包装 | pkg of 1 mg |
| 储存条件 | avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.) |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | endotoxin, tested |
| web metadata keyword.default | ketatinocyte, KAF, recombinant human |
| UniProt登记号 | P15514 |
| 储存温度 | −20℃ |
| Gene Information | human ... AREG(374) |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
Sigma-Aldrich
m.cnreagent.com
