产品说明
细胞系来源
Mouse C57BL Lewis lung carcinoma
细胞系描述
Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.
应用
Tumourigenicity, Metastasis and chemotherapeutic agent studies
培养基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using mechanical technique (aspiration); 5% CO2; 37℃. Cells detach as aggregates and are therefore difficult to count.
其他说明
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产品性质
生物来源 | mouse lung |
生长模式 | Adherent |
核型 | Not specified |
形态学 | Not specified |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |
Sigma-Aldrich