产品说明
一般描述
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
特异性
Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG, Fc fragment, or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG and rat serum proteins to ensure minimal cross reactivity.
免疫原
Purified mouse IgG
应用
Anti-Mouse IgG (Fab specific)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a minimum dilution of 1:64. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil slides at a working antibody dilution of 1:200. For immunofluorescence, a working dilution of 1:200 was used in various studies. To study DNA synthesis in mouse two-cell stage embryos by BrdU antibody, mouse IgG (Fab specific)-FITC antibody dilution of 1:50 was used.
其他说明
Antibody adsorbed with human IgG and rat serum proteins.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
制备说明
Adsorbed to reduce background with human or rat samples
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD00162622 |
NACRES | NA.46 |
产品性质
质量水平 | 200 |
生物来源 | goat |
偶联物 | FITC conjugate |
抗体形式 | affinity isolated antibody |
antibody product type | secondary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
储存条件 | protect from light |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 indirect immunofluorescence: 1:64 |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich