SK MES 1

产品说明

细胞系来源

Human Caucasian lung squamous carcinoma

细胞系描述

Derived from the pleural effusion of a 65 year old Caucasian male with squamous cell carcinoma of the lung. HLA = A3, B7, B27, w30.

应用

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质