产品说明
一般描述
REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
应用
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
特点和优势
组分
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
单位定义
在74℃下,一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。
法律信息
本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中:美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of sigma-aldrich Co. LLC
基本信息
MDL编号 | MFCD01635818 |
NACRES | NA.55 |
产品性质
质量水平 | 200 |
形式 | liquid |
用途 | sufficient for 1000 reactions sufficient for 250 reactions sufficient for 2500 reactions |
特点 | Difficult Templates/Specialty Enzymes PCR dNTPs included: no hotstart: no |
浓度 | 1 unit/μL |
technique(s) | PCR: suitable |
颜色 | red |
input | purified DNA |
application(s) | agriculture |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
Sigma-Aldrich