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REDTaq® Genomic DNA Polymerase

REDTaq® 基因组 DNA 聚合酶

品牌
Sigma-Aldrich
货号
D2812
规格纯度
without MgCl2
参考价格
957.17 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
REDTaq® Genomic DNA Polymerase
REDTaq® 基因组 DNA 聚合酶
产品介绍:

产品说明

一般描述

REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.

应用

REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.

特点和优势

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing

组分

Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2

单位定义

在74℃下,一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

法律信息

本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中:美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of sigma-aldrich Co. LLC

基本信息

MDL编号MFCD01635818
NACRESNA.55

产品性质

质量水平200
形式liquid
用途 sufficient for 1000 reactions
sufficient for 250 reactions
sufficient for 2500 reactions
特点Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no
浓度1 unit/μL
technique(s)PCR: suitable
颜色 red
input purified DNA
application(s)agriculture
运输wet ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids

Sigma-Aldrich

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