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位置:首页 > 品牌 > Sigma-Aldrich > Anti-CNTNAP2 Antibody, clone 2D17 ZooMAb® Rabbit Monoclonal

Anti-CNTNAP2 Antibody, clone 2D17 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1079
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
-+
产品名称:
Anti-CNTNAP2 Antibody, clone 2D17 ZooMAb® Rabbit Monoclonal
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2D17 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Contactin-associated protein-like 2 (CNTNAP2). It targets an epitope within 17 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Contactin-associated protein-like 2 (CNTNAP2).

应用

Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNTNAP2 in Rat brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNTNAP2 in Human brain tissue lysate.
Affinity Binding Assay:: A representative lot of this antibody bound CNTNAP2 peptide with a KD of 3.7 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CNTNAP2 in human cerebral cortex tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CNTNAP2, clone 2D17 ZooMAb®, Cat. No. ZRB1079, is a rabbit recombinant monoclonal antibody that specifically targets CNTNAP2 and is tested for use in Affinity Binding Assay, Immunohistochemistry, and Western Blotting.

目标描述

Contactin-associated protein-like 2 (UniProt: Q9UHC6; also known as Cell recognition molecule Caspr2, CNTNAP2) is encoded by the CNTNAP2 (also known as CASPR2, KIAA0868) gene (Gene ID: 26047) in human. CNTNAP2 is a single-pass type I membrane glycoprotein protein of the neurexin family that is predominantly expressed in the nervous system. It mediates synaptic cell adhesion and is required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. It also plays a role in the formation of functional distinct domains that are critical for saltatory conduction of nerve impulses in myelinated nerve fibers. CNTNAP2 is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 28-1262), a transmembrane domain (aa 1263-1283), and a cytoplasmic domain (aa 1284-1331). Knockdown of CNTNAP2 in cortical cultures is shown to impair the development of spines. Mutations in CNTNAP2 gene are known to be associated with intellectual disability, epilepsy, schizophrenia, language disorders, and autism. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gdalyahu A, et al., (2015). PLoS ONE. 10(5); e0125633).

外形

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2D17, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt ~ 148.17 kDa
observed mol wt ~ 165 kDa
species reactivityhuman, rat
species reactivity (predicted by homology)mouse, monkey
包装antibody small pack of 25 μL
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型IgG
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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