Anti-phospho-CREB (Ser133) Antibody, clone 10E9

产品说明

一般描述

CREB is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2.

特异性

Recognizes phosphorylated CREB at Mr 46 kDa. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide

免疫原

KLH-conjugated, synthetic peptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse. Clone 10E9.

应用

Research Sub Category
Transcription Factors
ELISA: Recommended at 0.05 μg/mL by an independent laboratory.
Research Category
Epigenetics & Nuclear Function
Anti-phospho-CREB (Ser133) Antibody, clone 10E9 is a Mouse Monoclonal Antibody for detection of phospho-CREB (Ser133) also known as active transcription factor CREB & has been validated in WB, ELISA.

质量

Evaluated by western blot on whole cells lysates from rat PC12 cells treated with forskolin.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected phosphorylated CREB in whole cell lysates from HepG2 and PC12 cells treated with forskolin.

目标描述

46 kDa

外形

Purified mouse monoclonal IgG1κ from serum-free cell culture supernatant, lyophilized from 1 mL of PBS, 0.09% sodium azide, PEG, and sucrose. Lyophilized.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

储存及稳定性

Lyophilized: Stable for 1 year at -20ºC from date of receipt.
Rehydrated: Stable for 3 months at 2-8ºC.
Aliquot solution to store frozen and avoid repeated freeze thaw cycles.

分析说明

Control
Forskolin-treated PC12 cell lysate

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H302 + H332 - H311 - H412
预防措施声明	P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
危险分类	Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 1